ELISpot Human IL-8 (CXCL8), pre-coated (5 plates)

Product Overview

Application

The Human IL-8 (CXCL8) Enzyme-Linked ImmunoSpot (ELISpot) assay is a highly sensitive method used to detect and quantify individual cells that secrete specific cytokines or other soluble molecules. This assay is utilized in immunology to monitor cellular immune responses at the single-cell level and reliably detects and measures human IL-8 secretion by stimulated effector cells. Pre-coated kits are ready to use, reduce manual handling, and speed up research workflows.

 

Description

Interleukin-8 (IL-8 / CXCL8) is a pro-inflammatory chemokine that plays a key role in the recruitment and activation of immune cells, particularly neutrophils. It is secreted by multiple cell types, including monocytes, macrophages, epithelial cells, and endothelial cells, in response to inflammatory stimuli or microbial products.

IL-8 is best known for directing neutrophil chemotaxis to sites of infection or tissue injury and enhancing their activation and degranulation. In addition to its role in innate immunity, IL-8 can also act on subsets of T cells, particularly memory and activated T cells that express CXCR1 or CXCR2 promoting their migration into inflamed tissues and contributing to the shaping of local immune responses.

Due to its role in immune cell recruitment, inflammation, and tissue remodeling, IL-8 is widely used as a biomarker in studies of infection, inflammation, and tumor immunology.

 

 

£845.00

Product Details

Application

ELISpot

Analyte

IL-8

Reactivity

Human

Specificity

Storage

Store plates and reagents between 2 and 8°C

Shelf Life

12 months

Kit Contents

Peptide Pools

No peptide pool included – plate and reagents only

Plates

ELISpot plate pre-coated with IL-8 capture antibody

Detection mAb

Biotinylated recombinant IL-8 antibody

Enzyme conjugate

Streptavidin-ALP (Alkaline Phosphatase)

Substrate

BCIP/NBT Ready-to-use solution

Blocking agent

Bovine Serum Albumin (BSA)