ELISpot Human IFN-γ

Product Details

Application

The Human IFN-γ Enzyme-Linked ImmunoSpot (ELISpot) assay is a highly sensitive method used to detect and quantify individual cells that secrete specific cytokines or other soluble molecules. This assay is utilized in immunology to monitor cellular immune responses at the single-cell level and reliably detects and measures human IFN-γ secretion by stimulated effector cells.

 

Description

IFN-γ is a dimerized soluble cytokine involved in both innate and adaptive immunity. It is produced primarily by Natural Killer cells during the innate immune response, and by CD4+ Th1 cells and CD8+ T cells during antigen-specific immune responses.

As a proinflammatory cytokine, IFN-γ plays a significant role in activating macrophages and endothelial cells, enhancing their pathogen-killing capabilities. It regulates immune responses by modulating the activity of antigen-presenting cells and T cells. IFN-γa is critical for both innate and adaptive immunity against viral, bacterial, and protozoal infections due to its ability to inhibit viral replication directly and through its immunostimulatory and immunomodulatory effects. One of its key functions is the induction of MHC class II molecule expression, which improves antigen presentation and immune recognition.

$247.00$975.00

Product Specifications

Product

ELISpot Human IFN-γ kit

Application

ELISPOT test: to detect and quantify individual cytokine-secreting cells, to understand the response of the adaptive immunity to a specific infection.

Analyte

IFN-γ

Reactivity

Human

Specificity

Human IFN-γ.

Storage

Store plates and reagents between 2 and 8°C.

Shelf life

18 months from date of receipt.

Kit content

Peptide pools

No peptide pool included – plate and reagents only

Plate

ELISpot plate precoated with IFN-γ capture antibody

Detection mAb

Biotinylated recombinant IFN-γ antibody

Enzyme conjugate

Streptavidin-ALP (Alkaline Phosphatase)

Substrate

BCIP/NBT Ready-to-use solution

Blocking agent

Bovine Serum Albumin (BSA)

Similar products