Avian Influenza (H5N1) ELISpot Human Collection Kit

Product Details

Application

The Avian Influenza Enzyme-Linked ImmunoSpot (ELISpot) Human Kit is a highly sensitive method used to detect and quantify individual cells that secrete IFN-γ, IL-2, and TNF-α after stimulation with the ImmuneSelect Avian Influenza Peptide Pool. This assay is utilized in immunology to monitor cellular immune responses at the single-cell level and reliably detects and measures cytokine secretion by stimulated effector cells.

£1,105.00£4,420.00

Product Specifications

Product

ViraxImmune H5N1 ELISpot Human kit collection

Application

ELISpot

Analyte

IFN-γ, IL-2, TNF-α

Reactivity

Human

Specificity

Human IFN-γ.

Storage

Store plates and reagents between 2 and 8°C. Peptide pool vial must be stored at -20 °C or below.

Shelf life

18 months from date of receipt.

Kit content

Peptide pools

ImmuneSelect H5N1 (55 peptides covering immunodominant MHC-I and MHC-II epitopes)

Plate

1 x ELISpot plate precoated with IFN-γ capture antibody, 1 x ELISpot plate precoated with IL-2 capture antibody, 1 x ELISpot plate precoated with TNF-α capture antibody,

Detection mAb

3 x vials of biotinylated recombinant mAbs: IFN-γ, IL-2, TNF-α

Enzyme conjugate

Streptavidin-ALP (Alkaline Phosphatase)

Substrate

BCIP/NBT Ready-to-use solution

Blocking agent

Bovine Serum Albumin (BSA)

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